Nefario easily overcome him using a fart gun and Lucy's lipstick-taser. El Macho then takes the mutagen himself, but Gru and Dr. Nefario made, whereupon they revert to their friendly yellow state. Nefario, Gru and his team spray all of the mutated minions with awful-tasting jelly containing a powerful antidote that Dr. He rescues the girls from the mutated minions & takes them to fight El Macho. Dr Nefario decides to go back to Gru's lab & develops an antidote for the PX41.

In order to rescue Lucy, Gru visits El Macho along with two minions covered in purple cupcake frosting, pretending he was captured by two of the mutant creatures. She is captured by El Macho, who knows she is an AVL agent after Pollito, his pet chicken, retrieves her AVL ID badge from her purse. On her flight to Australia, Lucy realizes she has feelings for Gru, so she jumps out of the plane and hang-glides down to the party. For breaking Margo's heart, Gru uses his freeze-ray to freeze Antonio in a block of ice. El Macho emphasizes that he and Gru could team up and they would become evil again followed by ruling the city, but Gru avoids answering and leaves with his daughter Margo, who in the meantime has broken up with Eduardo's two-timing son, Antonio, who had left Margo for another girl.

El Macho plans to send rockets full of mutated Minions to major cities and achieve world domination. Nefario) have captured and mutated a large number of Gru's minions using the PX-41, turning them into insane, savage, purple-furred monsters. Gru discovers that Eduardo (and his helper, Dr. Instead, he brings the girls to Eduardo's Cinco De Mayo party and finds proof that the Mexican restaurateur is in fact the super-villain El Macho. This leaves Gru heartbroken because he loves her, but he cannot find the courage to ask her out on a date. Before leaving, Lucy gives Gru her lipstick taser to remind him of her. The investigation is closed, and Lucy is reassigned to Australia. The next day, the AVL arrests Floyd Eagle-San (who claims that he was framed) because an almost-empty mutagen jar was found in his shop. They take Shannon home, and after they say good night, Gru realizes that Agnes was right: he has romantic feelings for Lucy. Luckily, Lucy bumps into them and rescues him from the date by shooting Shannon with a tranquilizer dart. After the investigation, Gru is set up on a horrible dinner date with a woman named Shannon, who notices Gru's wig and threatens to humiliate him. Gru and Lucy investigate wig merchant Floyd Eagle-San, but Gru is still suspicious of Eduardo. Meanwhile Margo meets Eduardo's son Antonio in the mall & has a huge crush on him. Gru tells her that his relationship with Lucy is strictly professional. Meanwhile, Agnes expresses her wish of having a mother someday and suspects that Gru will fall in love with Lucy. Gru and Lucy break into Eduardo's restaurant at night, but only find a jar of secret-recipe salsa and nothing to prove that Eduardo is the culprit. Gru suspects Mexican restaurant owner Eduardo Perez of being a super-villain called 'El Macho', a bad guy who became Gru's inspiration for a villain who supposedly died after sky-surfing a TNT-laden shark into the center of an active volcano. AVL also gives Gru and Lucy a list of shop owners whom they think are behind the theft. Gru reluctantly partners with undercover AVL agent Lucy Wilde, and together they search The Paradise Shopping Mall (where traces of PX41 have been spotted), where they are given a bakery called 'Bake My Day' as their headquarters. Nefario, his friend and assistant, has decided to leave him for new employment, because he 'missed being evil.' In his new avatar, Gru had Nefario produce a new line of Jams and Jellies, a task that Dr Naferio was terrible at. The Anti-Villain League (AVL) recruits former super-villain Gru (Steve Carell), now a devoted father to Margo, Edith and Agnes, to find out which evil person stole the lab, (which contained a powerful mutagen known as PX-41 which can make indestructible and extremely aggressive monsters out of living organisms) because Gru, being an ex-villain knows how villains work but refuses. A secret laboratory near the Arctic Circle is stolen by a mysterious vehicle using a giant magnet.

Despicable me 2